Terminator

Part:BBa_K5409711:Design

Designed by: Zhou Long   Group: iGEM24_SCUT-China-S   (2024-09-30)


RPL3t(Terminators of yeast)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to obtain RPL3t in yeast, we first need to obtain the genome information of RPL3. The gene information of RPL3 can be retrieved at https://yeastgenome.org (SGD ID: S000005589). After downloading the target information(.fasta), identify the target ORF (open reading frame) and select the 400bp DNA sequence after the stop codon as our RPL3t terminator sequence.

Tips: Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; plays an important role in function of eIF5B in stimulating 3' end processing of 18S rRNA in context of 80S ribosomes that have not yet engaged in translation; involved in replication and maintenance of killer double stranded RNA virus.

Source

RPL3 is come from Saccharomyces Genome Database (SGD) .Website :"https://yeastgenome.org/". SGD ID: S000005589

References